Polyclonal Antibody to Ubiquitin (Ub)

Ubiquitin B; UbB; Ubiquitous Immunopoietic Polypeptide; Polyubiquitin B

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
Quantity

Add to Cart Distributors

Overview

Properties

Product No.PAA164Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryReproductive science Neuro science Developmental science

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA164Hu01-Recombinant Ubiquitin (Ub)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

Share your citation Upload your experimental result Review Leave a message

Sign into your account

Email *

Password *

Verification code*

Create a new account

Share a new citation as an author

Title *

Journal

Link*

Upload your experimental result

Application*

Experimental result*

Review

Rating

Serial No* Please attach serial No. on instruction manual

Contact us
Please fill in the blank.

Name*

Organization

Address

E-mail address*

Telephone

Inquiry*

Verification code* CheckCode

Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Glioma Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human Ub Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Ub Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Ub Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Ub Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Ub Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Pancreatic cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human Ub Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Small intestine Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human Ub Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human Ub Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Western Blot; Sample: Human Lung lysate; Primary Ab: 1µg/ml Rabbit Anti-Human Ub Antibody Second Ab
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human Ub Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: Rat Cerebrum lysate; Lane3: MDA-MB-435S cell lysate;
Primary Ab: 0.1µg/ml Rabbit Anti-Human Ub Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against Ub. It has been selected for its ability to recognize Ub in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Increment services

Citations

Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancerPubmed: 32601970
Systemic ablation of vitamin D receptor leads to skeletal muscle glycogen storage disorder in mice34877816