Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) Homo sapiens (Human) Polyclonal antibody

KARP1; KU80; KUB2; Ku86; NFIV; TLAA; Lupus Ku Autoantigen Protein P80; Nuclear factor IV; 86 kDa of Ku antigen; CTC box-binding factor 85 kDa; Thyroid-lupus autoantigen

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPD013Hu01-Recombinant X-Ray Repair Cross Complementing 5 (XRCC5)
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) Packages (Simulation)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) Packages (Simulation)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) DAB staining on IHC-P;
    Samples: Human Prostate cancer Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) DAB staining on IHC-P;
    Samples: Human Cerebrum Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) DAB staining on IHC-P;
    Samples: Human Colorectal cancer Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) DAB staining on IHC-P;
    Samples: Human Liver Tissue;
    Primary Ab: 30µg/ml Rabbit Anti-Human XRCC5 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) FITC staining on IF;
    Samples: Human HepG2 Cells;
    Primary Ab: 20µg/ml Rabbit Anti-Human XRCC5 Antibody
    Second Ab: 1µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • PAD013Hu01.jpg WB Image
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human XRCC5 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) Western Blot; Sample: Human Liver lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human XRCC5 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) Western Blot; Sample: Human Lung lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human XRCC5 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human XRCC5 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to X-Ray Repair Cross Complementing 5 (XRCC5) Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human XRCC5 Polyclonal Antibody (Catalog PAD013Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against XRCC5. It has been selected for its ability to recognize XRCC5 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Non-canonical role for Ku70/80 in the prevention of allergic airway inflammation via maintenance of airway epithelial cell organelle homeostasisPubmed: 32877223

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