Recombinant Antibody to Cluster Of Differentiation 146 (CD146)

MCAM; MUC18; Melanoma Cell Adhesion Molecule; Cell surface glycoprotein P1H12; Melanoma-associated antigen A32; S-endo 1 endothelial-associated antigen

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 180.00 US$ 420.00 US$ 600.00 US$ 1,500.00 US$ 6,000.00
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Overview

Properties

Product No.RAB670Hu21
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IF; ICC; IHC; IP; FCM.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
Downloadn/a
CategoryCD & Adhesion molecule Tumor immunity Dermatology

SourceRecombinant monoclonal antibody preparation, Host Mouse
Ig Isotype IgG, Clone Number n/a
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen n/a
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 1mg/mL

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Packages (Simulation)
Packages (Simulation)

Human Hela cell line was stained with 5-10µg/ml mouse Anti-human CD146 Recombinant Antibodies (Catalog # RAB670Hu21, red histogram) or Isotype control antibody (Catalog # IS067-Mu02, green histogram), followed by 1µg/ml PE-conjugated Anti-mouse IgG Secondary Antibody (Catalog # SAA544Mu14).
Human peripheral blood lymphocytes were stained with 1µg/ml mouse Anti-human CD146 Recombinant Antibody (Catalog RAB670Hu21, green histogram) or Isotype control antibody (Catalog IS067-Mu03, blue histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CD146. It has been selected for its ability to recognize CD146 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:10μg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Citations

Evaluation of various endothelial biomarkers in ankylosing spondylitisPubMed: 21556780
The Effect of Regular Colchicine Treatment on Biomarkers Related with Vascular Injury in Newly Diagnosed Patients with Familial Mediterranean FeverSpringerLink: m7128503g576w808
Selective targeting of liver cancer with the endothelial marker CD146Pubmed:25238265
Melanoma cell adhesion molecule as an emerging biomarker with prognostic significance in systolic heart failurepubmed:27355366