Recombinant Antibody to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

CD152; CELIAC3; CTLA4; GSE; IDDM12; Celiac Disease 3; Cytotoxic T-lymphocyte protein 4

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Overview
Properties
  • SourceRecombinant monoclonal antibody preparation, Host CHO
  • Ig Isotype IgG, Clone Number n/a
  • PurificationProtein A + Protein G affinity chromatography
  • LabelBiotin
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Recombinant Antibody to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Packages (Simulation)
  • Recombinant Antibody to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Packages (Simulation)
  • Recombinant Antibody to Cytotoxic T-Lymphocyte Associated Antigen 4 (CTLA4) Briefly, biotin labelled CTLA4 Nano-antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to rh CTLA4-coated microtiter wells and incubated for 1h at 37℃. Wells were washed 3 times with PBST and incubated for 0.5 h at 37℃ with SA labelled HRP. After incubation with SA labelled HRP, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of rh CTLA4 and CTLA4 Nano-antibody was shown in Figure 1, and the ED50 for this effect is 0.06607 ug/ml.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CTLA4. It has been selected for its ability to recognize CTLA4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Circulating CTLA-4 levels and CTLA4 polymorphisms associate with disease condition and progression and hepatocellular carcinoma patients' survival in chronic …Pubmed: 32163858

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