Recombinant Antibody to Protein Tyrosine Phosphatase Receptor Type C (CD45)
PTPRC; PTPR-C; LCA; B220; GP180; LY5; T200; Leukocyte common antigen
- UOM
- FOB US$ 174.00 US$ 406.00 US$ 580.00 US$ 1,450.00 US$ 5,800.00
- Quantity
Overview
Properties
- Product No.RAB030Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; FCM.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - Downloadn/a
- CategorySignal transductionTumor immunityInfection immunity
- SourceRecombinant monoclonal antibody preparation, Host Mouse
- Ig Isotype IgG2b Kappa, Clone Number n/a
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPB030Hu01-Recombinant Protein Tyrosine Phosphatase Receptor Type C (CD45)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid, Concentration 0.1-1mg/mL
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Specifity
The antibody is a mouse monoclonal antibody raised against CD45. It has been selected for its ability to recognize CD45 in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Flow cytometry: 0.2-2 µL/106 cells
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Mouse Antibody Variable Region Gene Amplification Kit
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Real Time PCR Experimental Service
Citations
- Size-dependent segregation controls macrophage phagocytosis of antibody-opsonized targetsPubmed:29958103