Recombinant Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Homo sapiens (Human) Protein, Antibody, Assaykit, Service

T3E; TCRE; CD3-E Molecule,Epsilon(CD3-TCR Complex); T-cell surface antigen T3/Leu-4 epsilon chain

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Overview
Properties
  • SourceRecombinant monoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG, Clone Number n/a
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Recombinant Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Packages (Simulation)
  • Recombinant Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Packages (Simulation)
  • RAD117Hu21.jpg Figure. Western Blot; Sample: Recombinant CD3e, Human.
  • Recombinant Antibody to T-Cell Surface Glycoprotein CD3 Epsilon (CD3e) Human peripheral blood lymphocytes were stained with 10µg/ml mouse Anti-human CD3e Recombinant Antibodies (Catalog # RAD117Hu21, green histogram) or Isotype control antibody (Catalog # IS067-Mu03, blue histogram), FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog # SAA544Mu18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CD3e. It has been selected for its ability to recognize CD3e in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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