Recombinant Antibody to Tissue Factor Pathway Inhibitor (TFPI)

TFI; EPI; LACI; TFPI1; Lipoprotein-Associated Coagulation Inhibitor; Extrinsic pathway inhibitor

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 228.00 US$ 532.00 US$ 760.00 US$ 1,900.00 US$ 7,600.00
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Overview

Properties

Product No.RAA394Hu64
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IF; ICC; IHC; IP; FCM.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
Downloadn/a
CategoryMetabolic pathway Hematology

SourceRecombinant monoclonal antibody preparation, Host E.coli
Ig Isotype IgG, Clone Number n/a
PurificationProtein A + Protein G affinity chromatography
LabelCy3
Immunogen n/a
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 1mg/mL

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Packages (Simulation)
Packages (Simulation)

Briefly, recombinant TFPI antibody were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to recombinant human TFPI-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with HRP labelled secondary antibody wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450/630nm immediately. The binding activity of rh TFPI and recombinant TFPI antibody was shown in Figure 1, and this effect was in a dose dependent manner.

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against TFPI. It has been selected for its ability to recognize TFPI in immunohistochemical staining.

Usage

Immunocytochemistry: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Increment services

Citations

Tissue factor pathway inhibitor in paediatric patients with nephrotic syndromeSajch: 383
Cathepsin G in Experimental Tuberculosis: Relevance for Antibacterial Protection and Potential for ImmunotherapyPubMed: 26320257
Trans‐fatty acid promotes thrombus formation in mice by aggravating antithrombogenic endothelial functions via Toll‐like receptorsPubMed: 25546502
Endothelial cell‐anchored tissue factor pathway inhibitor regulates tumor metastasis to the lung in micePubMed: 25945811
Extracellular vesicles from malignant effusions induce tumor cell migration: inhibitory effect ofLMWH tinzaparin.pubmed:27435911
Kunitz-type protease inhibitor as a vaccine candidate against schistosomiasis mansonipubmed:29128645