Recombinant Perforin 1 (PRF1) 
FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein
- UOM
- FOB US$ 220.00 US$ 550.00 US$ 1,100.00 US$ 3,300.00 US$ 8,250.00
- Quantity
Overview
Properties
- Product No.RPB317Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Research use only - DownloadInstruction Manual
- CategoryImmune molecule
- Source Prokaryotic expression, Host E.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationMembrane, Secreted, Cytoplasm, Endosome
- Molecular Mass 65.3kDa, Accurate 65kDa(Analysis of differences refer to the manual)
- Residues & TagsVal40~Lys355 with N-terminal His and GST Tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
- Traits Freeze-dried powder, Purity > 90%
- Isoelectric Point7.4
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Packages (Simulation)
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Packages (Simulation)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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ISO9001: 2008, ISO13485: 2003 Registered
Sequence
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Storage
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Increment services
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Endotoxin Removal Kit
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BCA Protein Quantification Kit
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Protein Labeling Customized Service
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Molecular Mass Marker for Protein
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Recombinant Protein Customized Service
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Monoclonal Antibody Customized Service
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Polyclonal Antibody Customized Service
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Protein Activity Test Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Buffer
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Endotoxin Removal Kit II
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Real Time PCR Experimental Service
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
Citations
- Granzyme B as a diagnostic marker of tuberculosis in patients with and without HIV coinfectionPubmed:26915636
- The immunoregulatory effects of CD8 T-cell–derived perforin on diet-induced nonalcoholic steatohepatitisPubmed: 30951375
- A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumorsPubmed: 31695790
- CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protectionPubmed: 33223223
- Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumorsPubmed: 32772342
- Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma34377237
- Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy34873307
- Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusionPubmed:35061012