ELISA Kit DIY Materials for Clara Cell Protein 16 (CC16) Rattus norvegicus (Rat) Assay Kit DIY Materials

SCGB1A1; CC10; CCSP; UGB; UG; Uteroglobin; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, lung lavage fluid, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range78-5000pg/mL
  • Applicable Sensitivity30pg/mL
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  • ELISA Kit DIY Materials for Clara Cell Protein 16 (CC16) Packages (Simulation)
  • ELISA Kit DIY Materials for Clara Cell Protein 16 (CC16) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Clara Cell Protein 16 (CC16).No significant cross-reactivity or interference between Clara Cell Protein 16 (CC16) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Pulmonary functions and blood biochemical markers for workers with and without coal worker pneumoconiosisSciencepub: Source
  • Analysis of the Protective Biochemical and Pathologic Effects of Aminoguanidine on an Experimental Aspiration Pneumonitis Model Induced by Bile AcidsPubMed: S0011393X12000562
  • Effects of leflunomide on inflamation and fibrosis in bleomycine induced pulmonary fibrosis in wistar albino ratsPubmed: 24255778
  • The therapeutic effects of anti-oxidant and anti-inflammatory drug quercetin on aspiration-induced lung injury in ratsPubmed: 24122261
  • The effects of α-tocopherol on oxidative damage and serum levels of Clara cell protein 16 in aspiration pneumonitis induced by bile acidsPubmed: 23023024
  • The evaluation of different treatment protocols for trauma-induced lung injury in ratsPubmed: 24605218
  • Susceptibility to COPD: Differential Proteomic Profiling after Acute SmokingPubmed:25036363
  • Urine proteome analysis by C18 plate–matrix-assisted laser desorption/ionization time-of-flight mass spectrometry allows noninvasive differential diagnosis …Pubmed:30024955
  • N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation.Doi: 10.1042/BSR20180858
  • Mechanical activation of TRPV4 channels controls albumin reabsorption by proximal tubule cells.
  • An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis
  • Effects of mammary nutrient demand on glucose kinetics of dairy cows in early lactation
  • Role of bone marrow-derived mesenchymal stem cells in alleviating pulmonary epithelium damage and extracellular matrix remodeling in a rat model of lung fibrosis …Pubmed: 32909452
  • The Protective Effects of Butorphanol on Pulmonary Function of Patients with Obesity Undergoing Laparoscopic Bariatric Surgery: a Double-Blind Randomized …Pubmed: 32535786
  • Elevated plasma levels of epithelial and endothelial cell markers in COVID-19 survivors with reduced lung diffusing capacity six months after hospital …Pubmed:35189887
  • Lung-Protective Effects of Lidocaine Infusion on Patients with Intermediate/High Risk of Postoperative Pulmonary Complications: A Double-Blind Randomized …Pubmed:35422611

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