ELISA Kit DIY Materials for Immunoglobulin A (IgA) Bos taurus; Bovine (Cattle) Assay Kit DIY Materials

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable Sampleserum, plasma, saliva and other biological fluids
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.78-50ng/mL
  • Applicable Sensitivity0.34ng/mL
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  • ELISA Kit DIY Materials for Immunoglobulin A (IgA) Packages (Simulation)
  • ELISA Kit DIY Materials for Immunoglobulin A (IgA) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Immunoglobulin A (IgA). No significant cross-reactivity or interference between Immunoglobulin A (IgA) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Sequence-Based Typing-Study on the Relationship Between Subclinical Mastitis and BoLA-DRB3.2* Allelic Polymorphism in Egyptian CowsIdosi: Source
  • EVALUATION OF PHYSICO-CHEMICAL PROPERTIES OF COLOSTRUM SUPPLEMENTED DAHIIjfans: Source
  • Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88Pubmed: 24570422
  • Specific humoral immune response induced by Propionibacterium acnes can prevent Actinobacillus pleuropneumoniae infection in miceAsm: Source
  • Speci?c Humoral Immune Response Induced by Propionibacterium acnes Can Prevent Actinobacillus pleuropneumoniae Infection in MicePubmed:24429068
  • Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infectionPubmed:24576640
  • Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic micePubmed:25308407
  • Oropharyngeal colostrum administration in extremely premature infants: an RCT.Pubmed:25624376
  • Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosisPubMed: 26217323
  • Effects of fermented cottonseed meal on growth performance, serum biochemical parameters, immune functions, antioxidative abilities, and cecal microflora in broilers10.108:09540105.2017.1311308
  • SYSTEMISCHE ENTZÜNDUNG BEI CHRONISCHER NIERENINSUFFIZIENZ – DIE ROLLE DER DARMFLORApatent:US9763998B2
  • Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse modelPubmed:29655095
  • Recombinant Lactococcus lactis co-expressing OmpH of an M cell-targeting ligand and IBDV-VP2 protein provide immunological protection in chickensPubmed:29289381
  • Effects of and on growth performance, immune function and volatile fatty acid level of caecal digesta in broilers10.1080:09540105.2018.1457013
  • Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a …Pubmed:29305686
  • Altered immunoglobulins (A and G) in Ghanaian patients with type 2 diabetesPubmed:29623201
  • The gut flora modulates intestinal barrier integrity but not progression of chronic kidney disease in hyperoxaluria-related nephrocalcinosisPubmed: 31081025
  • Yacon (Smallanthus sonchifolius): Effect on Integrity of Intestinal Barrier, Inflammatory Response and Oxidative Stress in Animal Model of Colon Cancer (P06-052-19)Pubmed: 31225040
  • Effects of Paramylon Extracted from Euglena gracilis EOD-1 on Parameters Related to Metabolic Syndrome in Diet-Induced Obese MicePubmed: 31330894
  • Effects of polysaccharides from Yingshan Yunwu tea on meat quality, immune status and intestinal microflora in chickensPubmed: 32224178
  • Effect Long-Term Exposure to Different Doses of Lipopolysaccharides on the Intestinal Mucosal Immune Barrier
  • Effects of chitooligosaccharide supplementation on laying performance, egg quality, blood biochemistry, antioxidant capacity and immunity of laying hens during the …
  • Women Scientist Scientist Mentor
  • MicroRNA profiling in Chinese children with Henoch©\Schonlein purpura and association between selected microRNAs and inflammatory biomarkers33533510
  • Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure34090073
  • Monascus ruber fermented Panax ginseng ameliorates lipid metabolism disorders and modulate gut microbiota in rats fed a high-fat diet34098018
  • Dietary Supplementation of Inulin Ameliorates Subclinical Mastitis via Regulation of Rumen Microbial Community and Metabolites in Dairy Cows34494854
  • Sulfated modification enhances the immunomodulatory effect of Cyclocarya paliurus polysaccharide on cyclophosphamide-induced immunosuppressed mice through …34865774
  • Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein ComponentsPubmed:35438093
  • The effect of the multi-strain probiotic preparation EM Bokashi® on selected parameters of the cellular immune response in pigs
  • Bovine lactoferricin ameliorates intestinal inflammation and mucosal barrier lesions in colitis through NF-κB/NLRP3 signaling pathways
  • Salivary IgA Depression in Drug-Influenced Gingival Enlargement among Hypertensive Patients

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