ELISA Kit DIY Materials for Mucin 5 Subtype AC (MUC5AC)
TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtypes A And C,Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
- UOM
- FOB US$ 1,134.00 US$ 1,890.00 US$ 3,402.00 US$ 6,615.00 US$ 11,340.00
- Quantity
Overview
Properties
- Product No.KSA756Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - DownloadInstruction Manual
- CategoryTumor immunityInfection immunityEndocrinologyPulmonology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable Sampletissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range78-5,000pg/mL
- Applicable Sensitivity34pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Mucin 5 Subtype AC (MUC5AC). No significant cross-reactivity or interference between Mucin 5 Subtype AC (MUC5AC) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.PubMed: 20717734
- Entrapment of Conjunctival Goblet Cells by Desiccation-Induced CornificationPubMed: 21421863
- Role of aquaporin 5 in antigen-induced airway inflammation and mucous hyperproduction in micePubMed: 20550619
- Homeostatic control of conjunctival mucosal goblet cells by NKT-derived IL-13Nature: 201082a
- Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathwaysScienceDirect: S0014299912005523
- Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway InflammationPubMed: PMC3639905
- Pyocyanin-induced mucin production is associated with redox modification of FOXA2PubMed: PMC3765780
- A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC)Pubmed: 24468034
- The human cathelicidin LL-37 enhances airway mucus production in chronic obstructive pulmonary diseasePubmed:24291709
- Proteomic analysis of cancer and mesothelial cells reveals an increase in Mucin 5AC during ovarian cancer and peritoneal interactionPubmed:24726482
- The human Cathelicidin LL-37 induces MUC5AC mucin production by airway epithelial cells via TACE-TGF-α-EGFR pathwayPubmed:24901072
- Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significancePubmed:25151557
- Leukotriene E 4 induces MUC5AC release from human airway epithelial NCI-H292 cellsPubmed:25838093
- Secreted mucins and airway bacterial colonization in non-CF bronchiectasisPubMed: 26172851
- Isabela Casagrande JeremiasTeses: Disponiveis
- Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsivenessdoi:10.1164
- ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial CellsPubmed:27075970
- The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghaifiles:ijcem0020163.pdf
- Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonizationdoi:10.1513
- Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office WorkersPubmed:27297822
- Interleukin-33 induces mucin gene expression and goblet cell hyperplasia in human nasalepithelial cells.pubmed:27776277
- Mutating the CX3C Motif in the G Protein Should Make a Live Respiratory Syncytial Virus Vaccine Safer and More Effective.pubmed:28275196
- Peroxiredoxin 6 suppresses Muc5ac overproduction in LPS-induced airway inflammation through H2O2-EGFR-MAPK signaling pathway.pubmed:27884794
- Beneficial effects of prebiotic Saccharomyces cerevisiae mannan on allergic asthma mouse modelspubmed:28835901
- Efficacy of Sodium Hyaluronate in Murine Diabetic Ocular Surface Diseasespubmed:28644234
- Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression via the MKP-1-p38MAPK-dependent pathwaypubmed:28550735
- Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой10.20538/1682-0363-2017-2-146-158
- An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
- IL-4-induced caveolin-1-containing lipid rafts aggregation contributes to MUC5AC synthesis in bronchial epithelial cellspubmed:28931396
- Oxytetracycline Inhibits Mucus Secretion and Inflammation in Human Airway Epithelial Cells.pubmed:28578323
- Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polypsPubmed:29802623
- Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signalingPubmed:29382534
- Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in …Pubmed:29349683
- In vivo and in vitro investigation of anti-inflammatory and mucus-regulatory activities of a fixed combination of thyme and primula extractsPubmed:29698781
- Profiling of healthy and asthmatic airway smooth muscle cells following IL-1β treatment: a novel role for CCL20 in chronic mucus hyper-secretionPubmed:29946002
- Development of a highly sensitive and reliable enzyme-linked immunosorbent assay for MUC5aC in human tears extracted from schirmer stripsPubmed: 30214141
- Increased expression of long non-coding RNA SNHG16 correlates with tumor progression and poor prognosis in non-small cell lung cancerPubmed: 30287374
- The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung diseasePubmed: 30573494
- Regulation effects of naringin on diesel particulate matter-induced abnormal airway surface liquid secretionPubmed: 31301536
- Regional comparison of Goblet cell number and Area in exposed and covered Dry eyes and their Correlation with Tear MUC5ACPubmed: 32076085
- TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK …Pubmed: 33068589
- Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K …Pubmed: 32344236
- Less Airway Inflammation and Goblet Cell Metaplasia in an IL-33-Induced Asthma Model of Leptin-Deficient Obese Mice
- The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡33285209
- Porphyromonas gingivalis gingipains potentially affect MUC5AC gene expression and protein levels in respiratory epithelial cells33332733
- Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs33535050