ELISA Kit DIY Materials for Bradykinin (BK)
- UOM
- FOB US$ 905.00 US$ 1,508.00 US$ 2,714.00 US$ 5,278.00 US$ 9,048.00
- Quantity
Overview
Properties
- Product No.KSA874Ra11
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryEndocrinologyCardiovascular biologyPulmonologyHormone metabolism
- Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
- Detectable Range1.23-100pg/mL
- Applicable Sensitivity0.48pg/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Bradykinin (BK). No significant cross-reactivity or interference between Bradykinin (BK) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Bradykinin-Induced Shock Increase Exhaled Nitric Oxide, Complement Activation and Cytokine Production in Pigshandle:10037
- A transcriptomic study of Hereditary Angioedema attacksPubmed:29729940
- Bradykinin contributes to immune liver injury via B2R receptor-mediated pathways in trichloroethylene sensitized mice: a role in Kupffer cell activationPubmed: 30685357
- Increased expression of TRPV1 in patients with acute or chronic cough after lung cancer surgeryPubmed: 30883022
- Effects of nanoparticles on Neuroinflammation in a Mouse Model of AsthmaPubmed: 31542455