ELISA Kit DIY Materials for Interferon Alpha (IFNa) Homo sapiens (Human) Assay Kit DIY Materials

IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range7.8-500pg/mL
  • Applicable Sensitivity3.0pg/mL
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  • ELISA Kit DIY Materials for Interferon Alpha (IFNa) Packages (Simulation)
  • ELISA Kit DIY Materials for Interferon Alpha (IFNa) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Interferon Alpha (IFNa). No significant cross-reactivity or interference between Interferon Alpha (IFNa) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease VaccineMedwell: 2010610616
  • Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease VirusMedwell: 2010594600
  • The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and ratsPubMed: 23172753
  • Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatformPubMed: 23394132
  • Experimental rat model for alcohol-induced osteonecrosis of the femoral headPubMed: PMC3781777
  • A recombinant adenovirus bicistronically expressing porcine interferon-α and interferon-γ enhances antiviral effects against foot-and-mouth disease virusScienceDirect: S0166354214000266
  • Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cellsOnlinelibrary: bph.12440
  • High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor modelPubmed:24950740
  • Respiratory Mechanics and Plasma Levels of Tumor Necrosis Factor Alpha and Interleukin 6 Are Affected by Gas Humidification during Mechanical Ventilation in DogsPubmed:25036811
  • Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strainPubmed:25106750
  • Effect of SecinH3 on lung injury induced by sepsis of ratsPubMed: 26706678
  • siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In VitroPubmed:26886455
  • Sulforaphane Suppresses Hepatitis C Virus Replication by Up-ReCavia (Guinea pig )lating Heme Oxygenase-1 Expressionthrough PI3K/Nrf2 Pathway.Pubmed:27023634
  • Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in Cavia (Guinea pig )inea pigs.Pubmed:23776655
  • Impact of porcine reproductive and respiratory syndrome virus and porcine circovirus-2 infection on the potency of the classical swine fever vaccine (LOM strain).pubmed:27599928
  • Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cellsfiles:ijcep0036479.pdf
  • Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress.pubmed:27384769
  • Deoxyschizandrin suppresses dss-induced ulcerative colitis in mice.pubmed:27976641
  • MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis Bpubmed:28342861
  • The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infectionpubmed:28988058
  • Coinjection of a vaccine and anti-viral agents can provide fast-acting protection from foot-and-mouth diseasepubmed:28454913
  • Anti-high mobility group box 1 antibody ameliorates albuminuria in MRL/lprlupus-prone micepubmed:28649578
  • Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation.pubmed:28915824
  • Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposisPubmed:30056130
  • Role of Toll‐like receptor 9 signaling on activation of nasal polyp–derived fibroblasts and its association with nasal polypogenesisPubmed:29901280
  • Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosusPubmed: 30336754
  • Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in micePubmed: 30929267
  • Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens
  • Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in micePubmed: 31228813
  • Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1
  • Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in ChinaPubmed: 32187856
  • A flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in pigletsPubmed: 32253616
  • LDHB inhibition induces mitophagy and facilitates the progression of CSFV infectionPubmed: 32924761
  • Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasionPubmed: 32810792
  • BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in micePubmed: 32717566
  • Aquaporin‑4 deletion ameliorates enterovirus 71 infection in micePubmed: 32582978
  • Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon …
  • ГОРМОНАЛЬНЫЙ И ЦИТОКИНОВЫЙ ПРОФИЛЬ КРОВИ МОЛОЧНЫХ КОРОВ В РАННИЙ ПЕРИОД ГЕСТАЦИИ
  • Influenza virus neuraminidase regulates host CD8+ T-cell response in mice33293641
  • Concentration-Dependent Type 1 Interferon-Induced Regulation of MX1 and FABP3 in Bovine Endometrial Explants33494350
  • IFN©\¦Á and TNF©\¦Á serum levels and their association with disease severity in Egyptian children and adults with alopecia areata34008204
  • Orexin A Suppresses the Expression of Exosomal PD-L1 in Colon Cancer and Promotes T Cell Activity by Inhibiting JAK2/STAT3 Signaling Pathway34097168
  • Clinical findings of Talaromyces marneffei infection among patients with anti-interferon-¦Ã immunodeficiency: a prospective cohort study34144700
  • Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats34555716
  • The anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides34823813
  • Effects of different selenium sources on duodenum and jejunum tight junction network and growth performance of broilers in a model of fluorine-induced …Pubmed:35066382
  • Self-assembled BPIV3 nanoparticles can induce comprehensive immune responses and protection against BPIV3 challenge by inducing dendritic cell maturation in …Pubmed:35395543

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