ELISA Kit DIY Materials for Mucin 5 Subtype B (MUC5B)
MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin
- UOM
- FOB US$ 1,231.00 US$ 2,052.00 US$ 3,694.00 US$ 7,182.00 US$ 12,312.00
- Quantity
Overview
Properties
- Product No.KSA684Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryTumor immunityInfection immunityPulmonologyDermatology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.312-20ng/mL
- Applicable Sensitivity0.115ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Mucin 5 Subtype B (MUC5B). No significant cross-reactivity or interference between Mucin 5 Subtype B (MUC5B) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via D-Prostanoid Receptor SignalingJcb: 34199
- The ERK MAPK/RSK1 cascade phosphorylates CREB to induce MUC5B gene expression via DP1 receptor signalingJcb: 247684
- Pyocyanin-induced mucin production is associated with redox modification of FOXA2PubMed: PMC3765780
- Secreted mucins and airway bacterial colonization in non-CF bronchiectasisPubMed: 26172851
- Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsivenessdoi:10.1164
- Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonizationdoi:10.1513
- Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой10.20538/1682-0363-2017-2-146-158
- Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polypsPubmed:29802623
- Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signalingPubmed:29382534
- インフルエンザ菌Phosphorylcholine の表出と中耳ムチンMUC5AC 及びMUC5B 産生への影響10.5648:jjiao.36.7
- The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung diseasePubmed: 30573494
- ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanismPubmed: 31278375