ELISA Kit DIY Materials for Surfactant Protein B (SP-B)
SFTPB; PSPB; SFTB3; SFTP3; SPB; Surfactant Associated Protein B; Pulmonary Surfactant Protein B; 18 kDa pulmonary-surfactant protein; 6 kDa protein
- UOM
- FOB US$ 1,300.00 US$ 2,166.00 US$ 3,899.00 US$ 7,581.00 US$ 12,996.00
- Quantity
Overview
Properties
- Product No.KSB622Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
Research use only - Downloadn/a
- CategoryTumor immunityInfection immunityPulmonology
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates, cell lysates, lung lavage fluid and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.156-10ng/mL
- Applicable Sensitivity0.054ng/mL
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Specifity
The Abs in the kit have high sensitivity and excellent specificity for detection of Surfactant Associated Protein B (SPB). No significant cross-reactivity or interference between Surfactant Associated Protein B (SPB) and analogues was observed.
Usage
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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Citations
- Kinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgeryPubMed: 21736957
- Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polypsPubMed: 23406594
- Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot studyPubMed: 20650982
- Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant ProteinsPubMed: PMC3551896
- The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNSPubMed: PMC3787032
- Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor ModelsPubMed: PMC3406925
- Acute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps projectPubmed: 23619193
- Surfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart FailurePubmed:25514679
- Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene AugeOpus4:Source
- Plasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective studyPubMed: 26310985
- Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE)Pubmed:27337142
- The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions.pubmed:27656877
- Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid.pubmed:28101052
- Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal FluidPMC5209370
- Diving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional diverspubmed:28467885
- Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Productionpubmed:28743125
- Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency.pubmed:29167126
- Effects and molecular mechanisms of intrauterine infection/inflammation on lung developmentPubmed:29747649
- Surfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with LevosimendanPubmed:29548887
- Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD ExacerbationPubmed:29445934
- Generation of an alveolar epithelial type II cell line from induced pluripotent stem cellsPubmed: 30211653
- Culture of human alveolar epithelial type II cells by sproutingPubmed: 30340591