ELISA Kit DIY Materials for Toll Like Receptor 2 (TLR2) Mus musculus (Mouse) Assay Kit DIY Materials

CD282; TIL4; Toll/interleukin-1 receptor-like protein 4

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Overview
Properties
  • Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
  • Detectable Range0.312-20ng/mL
  • Applicable Sensitivity0.138ng/mL
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  • ELISA Kit DIY Materials for Toll Like Receptor 2 (TLR2) Packages (Simulation)
  • ELISA Kit DIY Materials for Toll Like Receptor 2 (TLR2) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The Abs in the kit have high sensitivity and excellent specificity for detection of Toll Like Receptor 2 (TLR2). No significant cross-reactivity or interference between Toll Like Receptor 2 (TLR2) and analogues was observed.

Usage

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

Storage

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

Support pack

Citations

  • Expression of soluble Toll-like receptors in pleural effusionsCMJ: 2010823483999506297
  • Baicalein Reduces Airway Injury in Allergen and IL-13 Induced Airway InflammationPubMed: PMC3639905
  • TLR2 in Pleural Fluid Is Modulated by Talc Particles during PleurodesisPubMed: PMC3529880
  • The Possible Role of TLR2 in Chronic Hepatitis B Patients with Precore MutationPubmed: 24187552
  • Cervical and vaginal fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranesPubmed:25028801
  • Cytokines and Toll-Like Receptors in the Immune Response to Influenza VaccinationPubmed:25248345
  • Correlation?between?salivary?Toll like receptor-2?concentration?and early childhood caries.Pubmed:25265998
  • Membrane and soluble Toll‐like receptor 2 in patients with psoriasis treated by Goeckerman therapyPubmed:25266302
  • The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseasesPubmed:25406630
  • Cathelicidin related antimicrobial peptide, laminin, Toll-like receptors and chemokines levels in experimental hypersensitivity pneumonitis in micePubMed: 25834936
  • Soluble Toll-Like Receptors 2 and 4 in Cerebrospinal Fluid of Patients with Acute Hydrocephalus followingAneurysmal Subarachnoid Haemorrhage.Pubmed:27223696
  • The Effect of Rosuvastatin on Markers of Immune Activation in Treatment-Naive Human Immunodeficiency Virus-PatientsPubmed:26835476
  • Pentraxin 3 and other inflammatory biomarkers related to atrial fibrillation in cardiac surgerypubmed:27852958
  • Soluble TLR2 and 4 concentrations in cerebrospinal fluid in HIV/SIV-relatedneuropathological conditions.pubmed:27882497
  • Toll-like receptors and their soluble forms differ in the knee and thumb basal osteoarthritic jointspubmed:28093922
  • Features of the Pattern Recognition Receptor TLR-2 Expression and Cytokines IL-2, IL-4 Profile in Children with Food Hypersensitivity10.12988:bmgt.2018.812
  • Prospective study on the significance of mucosal barrier dysfunction markers in assessing the effectiveness of the new strategy for treating food hypersensitivity in …10.22141:2224-0551.13.2.2018.129548
  • Vinpocetine regulates levels of circulating TLRs in Parkinson's disease patientsPubmed: 30315378
  • PROGNOSTIC ROLE OF IL-4, IL-2 AND TLR-2 FOR PREDICTING OF COURSE OF FOOD ALLERGY IN CHILDRENDoi: 10.29256/v.02.02.2018.escbm71
  • STUDY OF THE ACTION OF CHROMIUM-CONTAINING BIOPREPARATIONS FROM SPIRULINA ON INSULIN ACTIVITY IN RATSDoi: 10.29256/v.02.02.2018.escbm72
  • Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors-2 and-4
  • Aberrant DNA methylation of the toll-like receptors 2 and 6 genes in patients with obstructive sleep apneaPubmed: 32069296
  • ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE …Pubmed: 32242853
  • ДОбОвА вАРІАбЕЛЬНІСТЬ РІвНЯ РОзЧИННИХ ТОЛ-пОДІбНИХ РЕцЕпТОРІв 2 в СИРОвАТцІ КРОвІ ЖІНОК, ХвОРИХ НА РЕвМАТОїДНИй АРТРИТ: зв' …
  • FEATURES OF MYOCARDIAL REMODELING IN MEN WITH POST-INFARCTION CARDIOSCLEROSIS AND ITS RELATIONSHIP WITH TOLL-LIKE …
  • Levels of toll-like receptors 2 and 4 in serum of men with postinfarction cardiosclerosis and its relationship with antiphospholipid syndrome components
  • A value of soluble Toll‐like receptor 2 and 4 in vaginally obtained amniotic fluid for the prediction of histological chorioamnionitis34244993
  • Altered innate immune profile in blood of systemic mastocytosis patientsPubmed:35734269

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