Monoclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) Homo sapiens (Human) Monoclonal antibody

BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme; Alkaline Phosphatase, Liver/Bone/Kidney

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C4
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB091Hu01-Recombinant Alkaline Phosphatase, Tissue-nonspecific (ALPL)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) Packages (Simulation)
  • Monoclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) Packages (Simulation)
  • Monoclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) DAB staining on IHC-P; Sample: Human Liver Tissue; Primary Ab: 30µg/ml Mouse Anti-Human ALPL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) DAB staining on IHC-P;
    Sample: Human Liver Tissue
    Primary Ab: 30µg/ml Mouse Anti-Human ALPL Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAB091Hu22.jpg Figure. Western Blot; Sample: Recombinant ALPL, Human.
  • Monoclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) Western Blot; Sample: Lane1: Porcine Liver lysate; Lane2: Porcine Heart lysate; Lane3: Hela cell lysate
    Primary Ab: 2μg/ml Mouse Anti-Human ALPL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against ALPL. It has been selected for its ability to recognize ALPL in immunohistochemical staining and western blotting.

Usage

Western blottin:g: 0.01-2µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Osteopenic bone cell response to strontium-substituted hydroxyapatiteSpringerLink: n8233337w171v451
  • Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activationPubMed: 23400250
  • Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in miceWiley: Source
  • The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat modelPubmed:25544359
  • Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regenerationPubmed:25586067
  • Effect of Mirtazapine on Rat Bone Tissue after OrchidectomyPubMed: 25871861
  • New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization.Pubmed:27239050
  • MiR-142-5p promotes bone repair by maintaining osteoblast activityPubmed:27085967
  • New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological …doi:10.1002
  • MiR‑142‑5p promotes bone repair by maintaining osteoblast activitypubmed:27085967
  • Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasmaPubmed:30038263
  • Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In VivoPubmed:29562730
  • Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive RatsPubmed:29898457
  • Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice10.3892:etm.2018.6353
  • Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9
  • The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro
  • Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect34104195

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