Polyclonal Antibody to Apoptosis Signal Regulating Kinase 1 (ASK1)

MAP3-K5; MAP3K5; ASK1; MAPKKK5; MEKK5; Mitogen-Activated Protein Kinase Kinase Kinase 5; MAPK/ERK kinase kinase 5

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 100.00 US$ 232.00 US$ 332.00 US$ 830.00 US$ 3,320.00
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Overview

Properties

Product No.PAB358Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryEnzyme & Kinase Apoptosis

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB358Hu01-Recombinant Apoptosis Signal Regulating Kinase 1 (ASK1)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/ml

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Packages (Simulation)
Packages (Simulation)

IHC Images

Figure. Western Blot; Sample: Recombinant ASK1, Human.

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ASK1. It has been selected for its ability to recognize ASK1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

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Citations

Heat-shock protein 70 modulates apoptosis signal-regulating kinase 1 in stressed hepatocytes of Mugil cephalusPubmed:24875452
Differential reCavia (Guinea pig )lation of pro- and antiapoptotic proteins in fish adipocytes during hypoxic conditions.Pubmed:26744268
ASK-1 Expression Down-regulated in Preeclamptic Placental Explants Incubated with Mint-Tea:
Differential expression of survival proteins during decreased intracellular oxygen tension in brain endothelial cells of grey mullets.pubmed:28043644
Upregulation of HSP70 Extends Cytoprotection to Fish Brain under Xenobiotic Stressb0407ebde042c4f61b728bc11a586e6c