Polyclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) Homo sapiens (Human) Polyclonal antibody

ASP2; Beta Secretase; Memapsin 2; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1; Membrane-associated aspartic protease 2

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA718Hu01-Recombinant Beta-Site APP Cleaving Enzyme 1 (bACE1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.47mg/mL
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  • Polyclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) Packages (Simulation)
  • Polyclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) Packages (Simulation)
  • Polyclonal Antibody to Beta-Site APP Cleaving Enzyme 1 (bACE1) DAB staining on IHC-P; Samples: Human Spleen Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human bACE1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • PAA718Hu01.jpg Figure. Western Blot; Sample: Recombinant bACE1, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against bACE1. It has been selected for its ability to recognize bACE1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Comparative study of the effects of phosphatidylcholine rich in DHA and EPA on Alzheimer’s disease and the possible mechanisms in CHO-APP/PS1 cells and SAMP8 micepubmed:29292421
  • EPA enriched ethanolamine plasmalogens significantly improve cognition of Alzheimer's disease mouse model by suppressing β-amyloid generation10.1016/j.jff.2017.12.016
  • Mechanisms of DHA-enriched phospholipids in improving cognitive deficits in aged SAMP8 mice with high-fat dietPubmed:29986309
  • Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in micePubmed: 32522471
  • Effects of Alcohol Intake on Cognitive Function and ¦Â-amyloid Protein in APP/PS1 Transgenic Mice33737111

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