Polyclonal Antibody to Epithelial Cell Adhesion Molecule (EPCAM)

CD326; TACSTD1; EGP; KSA; M4S1; MIC18; TROP1; HEGP2; ESA; Epithelial glycoprotein; Adenocarcinoma-associated antigen; Tumor Associated Calcium Signal Transducer 1

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 100.00 US$ 232.00 US$ 332.00 US$ 830.00 US$ 3,320.00
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Overview

Properties

Product No.PAB283Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryCD & Adhesion molecule

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB283Hu01-Recombinant Epithelial Cell Adhesion Molecule (EPCAM)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.24mg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Colorectal cancer Tissue; Primary Ab: 10ug/ml Rabbit Anti-Human EPCAM Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Colorectal cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human EPCAM Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Western Blot; Samples: Lane1: HCT116 cell lysate; Lane2: A431 cell lysate; Lane3: MCF7 cell lysate; Primary Ab: 0.3µg/ml Rabbit Anti-Human EPCAM Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Porcine Colon lysate; Lane2: HCT116 cell lysate
Primary Ab: 1μg/ml Rabbit Anti-Human EPCAM Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against EPCAM. It has been selected for its ability to recognize EPCAM in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Increment services

Citations

Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platformScience: Article
Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determinationscience:S0026265X16300091
Role of several cytokines and adhesion molecules in the diagnosis and prediction of survival ofhepatocellular carcinoma.pubmed:27916547
Integrated bio-affinity nano-platform into a microfluidic immunosensor based on monoclonal bispecific trifunctional antibodies for the electrochemical determination of …pubmed:27836687
Integrated bio-affinity nano-platform into a microfluidic immunosensor based on monoclonal bispecific trifunctional antibodies for the electrochemical determination of epithelial cancer biomarker10.1016/j.cca.2016.11.012