Monoclonal Antibody to Epithelial Cell Adhesion Molecule (EPCAM) 
CD326; TACSTD1; EGP; KSA; M4S1; MIC18; TROP1; HEGP2; ESA; Epithelial glycoprotein; Adenocarcinoma-associated antigen; Tumor Associated Calcium Signal Transducer 1
Overview
Properties
- Product No.MAB283Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryCD & Adhesion molecule
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG1 Kappa, Clone Number C9
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPB283Hu01-Recombinant Epithelial Cell Adhesion Molecule (EPCAM)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 1mg/ml
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Packages (Simulation)
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Packages (Simulation)
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Western Blot; Sample: Porcine Kidney lysate Primary Ab: 2μg/ml Mouse Anti-Human EPCAM Antibody Second Ab: 0.2µg/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
Specifity
The antibody is a mouse monoclonal antibody raised against EPCAM. It has been selected for its ability to recognize EPCAM in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Organism Species More: Sus scrofa; Porcine (Pig)Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- Epithelial cancer biomarker EpCAM determination in peripheral blood samples using a microfluidic immunosensor based in silver nanoparticles as platformScience: Article
- Nanostructured platform integrated into a microfluidic immunosensor coupled to laser-induced fluorescence for the epithelial cancer biomarker determinationscience:S0026265X16300091
- Role of several cytokines and adhesion molecules in the diagnosis and prediction of survival ofhepatocellular carcinoma.pubmed:27916547
- Integrated bio-affinity nano-platform into a microfluidic immunosensor based on monoclonal bispecific trifunctional antibodies for the electrochemical determination of …pubmed:27836687
- Integrated bio-affinity nano-platform into a microfluidic immunosensor based on monoclonal bispecific trifunctional antibodies for the electrochemical determination of epithelial cancer biomarker10.1016/j.cca.2016.11.012