Polyclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)

HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; MAPK3; ERT2; Mitogen-Activated Protein Kinase 3; Insulin-stimulated MAP2 kinase; Microtubule-associated protein 2 kinase

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 110.00 US$ 258.00 US$ 368.00 US$ 920.00 US$ 3,680.00
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Overview

Properties

Product No.PAB357Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction Enzyme & Kinase Apoptosis Tumor immunity

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB357Hu01-Recombinant Extracellular Signal Regulated Kinase 1 (ERK1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Pancreatic cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human ERK1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Prostate cancer Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Cerebrum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human ERK1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Figure. Western Blot; Sample: Recombinant ERK1, Human.
Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Mouse Cerebrum lysate; Lane3: Mouse Heart lysate; Lane4: Porcine Liver lysate; Lane5: Porcine Cerebrum lysate
Primary Ab: 2µg/mL Rabbit Anti-Human ERK1 Ab
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ERK1. It has been selected for its ability to recognize ERK1 in immunohistochemical staining and western blotting.

Usage

Western blottin:g: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Sus scrofa; Porcine (Pig)

Giveaways

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Citations

Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndromepubmed:27538909
Gonadotropin Stimulation Has Only a Limited Effect on the Concentration of Follicular Fluid Signalling Proteins: An Antibody Array Analysis33575317
Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells34837026