Polyclonal Antibody to Glypican 4 (GPC4) Mus musculus (Mouse) Polyclonal antibody

K-Glypican; Glypican Proteoglycan 4; Secreted glypican-4

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA998Mu01-Recombinant Glypican 4 (GPC4)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Glypican 4 (GPC4) Packages (Simulation)
  • Polyclonal Antibody to Glypican 4 (GPC4) Packages (Simulation)
  • Polyclonal Antibody to Glypican 4 (GPC4) DAB staining on IHC-P; Samples: Mouse Pancreas Tissue; Primary Ab: 30µg/ml Rabbit Anti-Mouse GPC4 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Glypican 4 (GPC4) DAB staining on IHC-P;
    Samples: Mouse Pancreas Tissue
  • PAA998Mu01.jpg Figure. Western Blot; Sample: Recombinant GPC4, Mouse.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against GPC4. It has been selected for its ability to recognize GPC4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammationPubmed:Pmc4072672
  • Aspects of fluid therapy in the critically ill. Experimental and clinical studies on fluid therapy in the inflammatory conditions.250690_2_G5_Svajunas_S.pdf
  • Elevated plasma glypicans are associated with organ failure in patients with infectionPubmed: 30618011
  • Albumin infusion rate and plasma volume expansion: a randomized clinical trial in postoperative patients after major surgeryPubmed: 31138247
  • Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with …Pubmed: 32816247
  • Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study
  • Glypican-4 in pregnancy and its relation to glucose metabolism, insulin resistance and gestational diabetes mellitus status34903856

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