Polyclonal Antibody to Hypoxia Inducible Factor 2 Alpha (HIF2a)    
    
EPAS1; HLF; MOP2; PASD2; BHLHE73; Endothelial PAS Domain 1; HIF-1 Alpha-Like Factor; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73
Overview
Properties
- Product No.PAD466Mu01
 - Organism SpeciesMus musculus (Mouse) Same name, Different species.
 - ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
 - CategoryCardiovascular biology
 
- SourcePolyclonal antibody preparation, Host Rabbit
 - Ig Type IgG, Potency n/a
 - PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
 - LabelNone
 - Immunogen RPD466Mu01-Recombinant Hypoxia Inducible Factor 2 Alpha (HIF2a)
 - Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
 - TraitsLiquid, Concentration 500µg/mL
 
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Specifity
The antibody is a rabbit polyclonal antibody raised against HIF2a. It has been selected for its ability to recognize HIF2a in immunohistochemical staining and western blotting.
Usage
            Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.        
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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                    Antibody Labeling Customized Service
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                    Protein A/G Purification Column
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                    Staining Solution for Cells and Tissue
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                    Positive Control  for Antibody
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                    Tissue/Sections Customized Service
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                    Phosphorylated Antibody Customized Service
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                    Western Blot (WB) Experiment Service
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                    Immunohistochemistry (IHC) Experiment Service
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                    Immunocytochemistry (ICC) Experiment Service
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                    Flow Cytometry (FCM) Experiment Service
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                    Immunoprecipitation (IP) Experiment Service
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                    Immunofluorescence (IF) Experiment Service
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                    Buffer
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                    DAB Chromogen Kit
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                    SABC Kit
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                    Long-arm Biotin Labeling Kit
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                    Real Time PCR Experimental Service
                                 
Citations
- Combination viroimmunotherapy with checkpoint inhibition to treat glioma, based on location-specific tumor profilingPubMed: 26409567
 - Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trialissn:2542-3975
 - Telomere elongation protects heart and lung tissue cells from fatal damage in rats exposed to severe hypoxiaPubmed:29454386
 - Oxidative damage and iron dyshomeostasis in thalassemic patients
 - The Association Between Hypoxia Improvement and Electroconvulsive Therapy for Major Depressive Disorder34588778
 
