• KO-Validated
Polyclonal Antibody to Interleukin 7 Receptor (IL7R) Homo sapiens (Human) Polyclonal antibody

CDW127; CD127; IL-7R-Alpha

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB905Hu01-Recombinant Interleukin 7 Receptor (IL7R)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Interleukin 7 Receptor (IL7R) Packages (Simulation)
  • Polyclonal Antibody to Interleukin 7 Receptor (IL7R) Packages (Simulation)
  • Polyclonal Antibody to Interleukin 7 Receptor (IL7R) DAB staining on IHC-P; Samples: Human Stomach Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human IL7R An
  • Polyclonal Antibody to Interleukin 7 Receptor (IL7R) DAB staining on IHC-P;
    Samples: Human Skin cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human IL7R Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Interleukin 7 Receptor (IL7R) DAB staining on IHC-P;
    Samples: Human Liver Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human IL7R Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB905Hu01.jpg Figure. Western Blot; Sample: Recombinant IL7R, Human.
  • Polyclonal Antibody to Interleukin 7 Receptor (IL7R) Figure. Western Blot; Sample: Lane1: Human Lung Tissue; Lane2: Human K562 Cells; Lane3: Human Jurkat Cells.
  • Polyclonal Antibody to Interleukin 7 Receptor (IL7R) Knockout Varification:
    Lane 1: Wild-type K562 cell lysate;
    Lane 2: IL7R knockout K562 cell lysate;
    Predicted MW: 51,34,29kDa
    Observed MW: 60kDa
    Primary Ab: 2µg/ml Rabbit Anti-Human IL7R Ab
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IL7R. It has been selected for its ability to recognize IL7R in immunohistochemical staining and western blotting.

Usage

Western blotting: 1-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

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