• KO-Validated
Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Homo sapiens (Human) Polyclonal antibody

PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Packages (Simulation)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Packages (Simulation)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Used in DAB staining on fromalin fixed paraffin- embedded liver tissue
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) DAB staining on IHC-P;
    Samples: Human Liver Tissue
  • PAH839Hu01.jpg WB Image
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Knockout Varification:
    Lane 1: Wild-type HepG2 cell lysate;
    Lane 2: IDH1 knockout HepG2 cell lysate;
    Predicted MW: 47kDa
    Observed MW: 47kDa
    Primary Ab: 2µg/ml Rabbit Anti-Human IDH1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Western Blot; Sample: Mouse Liver lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human IDH1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Western Blot; Sample: Human Liver lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human IDH1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Western Blot; Sample: Porcine Liver lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human IDH1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human IDH1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IDH1. It has been selected for its ability to recognize IDH1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Citations

  • Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic AnalysisMcponline: 008821
  • Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic AnalysisPubmed: 22064513
  • Isocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancerPubmed: 24046070
  • The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cellsPubMed: 25914546

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