KO-Validated
Polyclonal Antibody to Karyopherin Alpha 2 (KPNa2)

IPOA1; QIP2; RCH1; SRP1alpha; RAG Cohort 1; Importin Alpha 1; RAG cohort protein 1; SRP1-alpha

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
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Overview

Properties

Product No.PAE748Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB,IHC,ICC/IF; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryImmune molecule

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPE748Hu01-Recombinant Karyopherin Alpha 2 (KPNa2)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/mL

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Packages (Simulation)
Packages (Simulation)

FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20µg/ml Rabbit Anti-Human KPNa2 Antibody Second Ab: 1.5µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
DAB staining on IHC-P;
Sample: Rat Lymph node Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human KPNa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Testis Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human KPNa2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Human KPNa2 Antibody
Second Ab: 2µg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant KPNa2, Human.
Western Blot; Sample: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: Raji cell lysate; Lane4: U2OS cell lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Human KPNa2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type HepG2 cell lysate;
Lane 2: KPNa2 knockout HepG2 cell lysate;
Predicted MW: 58kDa
Observed MW: 58kDa
Primary Ab: 3µg/ml Rabbit Anti-Human KPNa2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human KPNa2 Polyclonal Antibody (Catalog PAE748Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against KPNa2. It has been selected for its ability to recognize KPNa2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Flow cytometry:20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Increment services

Citations

KPNA2 is a promising biomarker candidate for esophageal squamous cell carcinoma and correlates with cell proliferationPubmed:25109899
KPNA2 is a potential diagnostic serum biomarker for epithelial ovarian cancer and correlates with poor prognosispubmed:28651492
Association of overexpressed karyopherin alpha 2 with poor survival and its contribution to interleukin‐1β‐induced matrix metalloproteinase expression in oral cancerPubmed:29542209
Nuclear transport proteins are secreted by cancer cells and identified as potential novel cancer biomarkers34591985

KO-Validated Polyclonal Antibody to Karyopherin Alpha 2 (KPNa2)