KO-Validated
Polyclonal Antibody to Cytokeratin 18 (CK18)

KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 100.00 US$ 232.00 US$ 332.00 US$ 830.00 US$ 3,320.00
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Overview

Properties

Product No.PAB231Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategoryTumor immunity Pulmonology Dermatology

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB231Hu01-Recombinant Cytokeratin 18 (CK18)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Samples: Human Ovary Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human KRT18 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human KRT18 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human KRT18 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human KRT18 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Kidney Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CK18 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Uterus Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human CK18 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CK18 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CK18 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Rat Uterus Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human CK18 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human KRT18 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Sample: Human Hela cell;
Primary Ab: 20ug/ml Rabbit Anti-Human CK18 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
FITC staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human CK18 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
FITC staining on IF;
Sample: Primary Mouse Neonatal Epidermal Keratinocytes cell
Primary Ab: 10µg/ml Rabbit Anti-Human CK18 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)

Figure. Western Blot; Sample: Recombinant KRT18, Human.
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: KRT18 knockout Hela cell lysate;
Predicted MW: 48kDa
Observed MW: 48kDa
Primary Ab: 2µg/ml Rabbit Anti-Human KRT18 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human KRT18 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Human KRT18 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against CK18. It has been selected for its ability to recognize CK18 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Increment services

Citations

Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosisPubmed:27186801
Cigarette Smoke-Induced Alterations in Frontal White Matter Lipid Profiles Demonstrated by MALDI-Imaging Mass Spectrometry: Relevance to Alzheimer's DiseasePubmed:26836183
Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring
Lipidomic‐based investigation into the therapeutic effects of PPC and BBD on rats with NAFLD34727379
Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …Pubmed:35212074

KO-Validated Polyclonal Antibody to Cytokeratin 18 (CK18)