Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Homo sapiens (Human) Polyclonal antibody

GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA698Hu01-Recombinant Macrophage Migration Inhibitory Factor (MIF)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Packages (Simulation)
  • Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Packages (Simulation)
  • Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human MIF Antib
  • Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human MIF Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA698Hu01.jpg Figure. Western Blot; Sample: Recombinant MIF, Human.
  • Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Western Blot; Sample: Human Liver lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human MIF Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Western Blot; Sample: Mouse Kidney lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human MIF Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human MIF Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MIF. It has been selected for its ability to recognize MIF in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Citations

  • Targeted reduction of advanced glycation improves renal function in obesityPubMed: 21412218
  • Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the GutJimmunol: 5673
  • Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver diseasePubMed: PMC3757527
  • Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing micePubMed: PMC3466372
  • Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetesPubmed:24957662
  • The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the BladderPubMed: 25705692
  • Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease.pubmed:28160092

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