Polyclonal Antibody to Perforin 1 (PRF1) Mus musculus (Mouse) Polyclonal antibody

FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB317Mu01-Recombinant Perforin 1 (PRF1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.23mg/mL
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  • Polyclonal Antibody to Perforin 1 (PRF1) Packages (Simulation)
  • Polyclonal Antibody to Perforin 1 (PRF1) Packages (Simulation)
  • Polyclonal Antibody to Perforin 1 (PRF1) Used in DAB staining on fromalin fixed paraffin-embedded spleen tissue
  • PAB317Mu01.jpg Figure. Western Blot; Sample: Recombinant PRF1, Mouse.
  • Polyclonal Antibody to Perforin 1 (PRF1) Western Blot; Sample: Mouse Liver lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Mouse PRF1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Perforin 1 (PRF1) Western Blot; Sample: Lane1: THP1 cell lysate; Lane2: U937 cell lysate; Lane3: Rat Lung lysate; Lane4: Rat Spleen lysate; Lane5: Mouse Spleen lysate
    Primary Ab: 0.2μg/ml Rabbit Anti-Mouse PRF1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PRF1. It has been selected for its ability to recognize PRF1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • Granzyme B as a diagnostic marker of tuberculosis in patients with and without HIV coinfectionPubmed:26915636
  • The immunoregulatory effects of CD8 T-cell–derived perforin on diet-induced nonalcoholic steatohepatitisPubmed: 30951375
  • A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumorsPubmed: 31695790
  • CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protectionPubmed: 33223223
  • Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumorsPubmed: 32772342
  • Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma34377237
  • Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy34873307
  • Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusionPubmed:35061012

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