Monoclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA)
PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide
Overview
Properties
- Product No.MAA528Hu22
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryCytokineTumor immunityInfection immunityBone metabolism
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG1 kappa, Clone Number C6
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA528Hu01-Recombinant Platelet Derived Growth Factor Subunit A (PDGFA)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 1mg/mL
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Specifity
The antibody is a mouse monoclonal antibody raised against PDGFA. It has been selected for its ability to recognize PDGFA in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy ratspubmed:28177680
- Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathwaypubmed:28521243
- Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells
- Lipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathwayPubmed: 31698141
- PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosisPubmed: 32208584
- Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method34339025
- Thermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like FormPubmed:35586817