Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB) Sus scrofa; Porcine (Pig) Polyclonal antibody

PDGF-B; PDGF2; SIS; SSV; C-sis; Simian Sarcoma Viral(v-sis)Oncogene Homolog; Platelet Derived Growth Factor Beta Polypeptide; Proto-oncogene c-Sis; Becaplermin

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC921Po01-Recombinant Platelet Derived Growth Factor Subunit B (PDGFB)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB) Packages (Simulation)
  • Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB) Packages (Simulation)
  • Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB) DAB staining on IHC-P; Samples: Porcine Cardiac Muscle Tissue; Primary Ab: 20µg/ml Rabbit Anti-Porcine PDGFB Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB) DAB staining on IHC-P;
    Samples: Porcine Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Porcine PDGFB Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB) DAB staining on IHC-P;
    Sample: Porcine Heart Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Porcine PDGFB Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB) DAB staining on IHC-P;
    Sample: Porcine Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Porcine PDGFB Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAC921Po01.jpg Figure. Western Blot; Sample: Recombinant PDGFB, Porcine.
  • Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB) Western Blot; Sample: Lane1: Porcine Serum; Lane2: Porcine Heart lysate; Lane3: Human Placenta lysate; Lane4: Porcine Pancreas lysate
    Primary Ab: 1μg/ml Rabbit Anti-Porcine PDGFB Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PDGFB. It has been selected for its ability to recognize PDGFB in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Citations

  • Inhibition of Glomerular Mesangial Cell Proliferation by siPDGF-B-and siPDGFR-β-Containing Chitosan Nanoplexespubmed:27975193
  • In vitro PDGF-B gene silencing studies and In vivo delivery of siRNA to the rat kidney using Chitosan/siRNA nanoplexes5000184338
  • Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagensISSN:1936-2625/IJCEP0058424
  • Melatonin promotes osteoblastic differentiation and regulates PDGF/AKT signaling pathwayPubmed: 31535749
  • PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosisPubmed: 32208584
  • Therapeutic potential of adipose-derived mesenchymal stem cell exosomes in tissue-engineered bladders33868627

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