• KO-Validated
Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) Homo sapiens (Human) Polyclonal antibody

CD265; TNFRSF11A; TNFRSF11-A; ODFR; OFE; TRANCER; Osteoclast differentiation factor receptor; Tumor Necrosis Factor Receptor Superfamily,Member 11a,NFKB Activator

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC057Hu01-Recombinant Receptor Activator Of Nuclear Factor Kappa B (RANk)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) Packages (Simulation)
  • Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) Packages (Simulation)
  • Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human RANk Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) DAB staining on IHC-P;
    Samples: Human Small intestine Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human RANk Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) DAB staining on IHC-P;
    Samples: Human Lymph node Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human RANk Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) DAB staining on IHC-P;
    Samples: Human Cerebrum Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human RANk Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) DAB staining on IHC-P;
    Samples: Human Cardiac Muscle Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human RANk Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) AF488 staining on IF;
    Sample: A431 cell
    Primary Ab: 20µg/ml Rabbit Anti-Human RANk Antibody
    Second Ab: 2µg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAC057Hu01.jpg Figure. Western Blot; Sample: Recombinant RANk, Human.
  • Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) Western Blot; Sample: Human 293T cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human RANk Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) Knockout Varification:
    Lane 1: Wild-type 293T cell lysate;
    Lane 2: RANk knockout 293T cell lysate;
    Predicted MW: 28,32,36,56,65kd
    Observed MW: 35kd
    Primary Ab: 1µg/ml Rabbit Anti-Human RANk Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B (RANk) Western Blot; Sample: Mouse Kidney lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human RANk Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against RANk. It has been selected for its ability to recognize RANk in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Giveaways

Citations

  • Human polymorphonuclear neutrophils express the receptor activator of NFκB (RANK) and are activated by its ligand RANKLWiley: source
  • Valvular osteoclasts in calcification and aortic valve stenosis severityPubMed: 23452891
  • Receptor Activator of Nuclear Transcription Factor NF-κB(RANK), Its Ligand RANKL, and Natural Inhibitor of RANKL Osteoprotegerin (OPG) in the Blood Serum of Patients with Primary Bone TumorsDOI:110.1007/s10517-017-3832-9
  • IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1α signaling pathwayPubmed: 32347300

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