Polyclonal Antibody to Spondin 2 (SPON2) Homo sapiens (Human) Polyclonal antibody

DIL1; Mindin; M-spondin; Extracellular Matrix Protein; Differentially expressed in cancerous and non-cancerous lung cells 1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPF396Hu01-Recombinant Spondin 2 (SPON2)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.45mg/mL
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  • Polyclonal Antibody to Spondin 2 (SPON2) Packages (Simulation)
  • Polyclonal Antibody to Spondin 2 (SPON2) Packages (Simulation)
  • Polyclonal Antibody to Spondin 2 (SPON2) DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human SPON2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Spondin 2 (SPON2) DAB staining on IHC-P;
    Samples: Human Stomach Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human SPON2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Spondin 2 (SPON2) DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human SPON2 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAF396Hu01.jpg Figure. Western Blot; Sample: Recombinant SPON2, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SPON2. It has been selected for its ability to recognize SPON2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Spondin-2, a secreted extracellular matrix protein, is a novel diagnostic biomarker for prostate cancerPubmed: 23665271
  • SP136EVALUATION OF SERUM SPONDIN 2 LEVELS IN THE DIFFERENT ETIOLOGIES OF GLOMERULAR DISEASESContent: 30
  • Evaluation of Serum Spondin 2 Levels in the Different Stages of Type 2 Diabetic NephropathyPubMed: 25973958
  • The extracellular matrix protein mindin attenuates colon cancer progression by blocking angiogenesis via Egr-1-mediated regulationpubmed:28991232
  • Study of human T21 placenta suggests a potential role of mesenchymal spondin-2 in placental vascular developmentPubmed: 30715257
  • Mindin serves as a tumour suppressor gene during colon cancer progression through MAPK/ERK signalling pathway in micePubmed: 32614521

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