Polyclonal Antibody to Syndecan 1 (SDC1) Homo sapiens (Human) Polyclonal antibody

CD138; SDC; SYND1; Syndecan Proteoglycan 1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB966Hu01-Recombinant Syndecan 1 (SDC1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Syndecan 1 (SDC1) Packages (Simulation)
  • Polyclonal Antibody to Syndecan 1 (SDC1) Packages (Simulation)
  • Polyclonal Antibody to Syndecan 1 (SDC1) DAB staining on IHC-P; Samples: Human Lung cancer Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human SDC1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Syndecan 1 (SDC1) DAB staining on IHC-P;
    Samples: Human Liver Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human SDC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Syndecan 1 (SDC1) DAB staining on IHC-P;
    Samples: Human Kidney Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human SDC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Syndecan 1 (SDC1) DAB staining on IHC-P;
    Samples: Human Pancreas Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human SDC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Syndecan 1 (SDC1) DAB staining on IHC-P;
    Samples: Human Colorectal cancer Tissue;
    Primary Ab: 20µg/ml Rabbit Anti-Human SDC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Syndecan 1 (SDC1) DAB staining on IHC-P;
    Sample: Human Placenta Tissue;
    Primary Ab: 10ug/ml Rabbit Anti-Human SDC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Syndecan 1 (SDC1) AF488 staining on IF;
    Sample: A431 cell
    Primary Ab: 20µg/ml Rabbit Anti-Human SDC1 Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAB966Hu01.jpg Figure. Western Blot; Sample: Recombinant SDC1, Human.
  • Polyclonal Antibody to Syndecan 1 (SDC1) Western Blot; Sample: Hela cell lysate
    Primary Ab: 0.5µg/ml Rabbit Anti-Human SDC1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Syndecan 1 (SDC1) Western Blot; Sample: Mouse Heart lysate
    Primary Ab: 0.4µg/ml Rabbit Anti-Human SDC1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against SDC1. It has been selected for its ability to recognize SDC1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Increased level of soluble syndecan-1 in serum correlates with myocardial expression in a rat model of myocardial infarctionSpringerLink: a201w3l18k257462
  • Sdc1 Overexpression Inhibits the p38 MAPK Pathway and Lessens Fibrotic Ventricular Remodeling in MI RatsSpringer: Source
  • Damage of the endothelial glycocalyx in chronic kidney diseasePubmed:24727235
  • Effect of valproic acid and injury on lesion size and endothelial glycocalyx shedding in a rodent model of isolated traumatic brain injuryPubmed:25058256
  • Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic RelevancePubmed:25692257
  • Dexamethasone Suppressed LPS-Induced Matrix Metalloproteinase and Its Effect on Endothelial Glycocalyx SheddingPubMed: 26199464
  • Platelet-Derived Growth Factor-B Protects Rat Cardiac Allografts From Ischemia-reperfusion Injury.PubMed: 26371596
  • Dual antiplatelet and anticoagulant APAC prevents experimental ischemia–reperfusion-induced acute kidney injurypubmed:27405618
  • Endothelial glycocalyx layer shedding following lung resectionpubmed:27643669
  • Resuscitation with Pooled and Pathogen-Reduced Plasma Attenuates the Increase in Brain Water Content following Traumatic Brain Injury and Hemorrhagic Shock in Rats10.1089
  • Resuscitation with pooled and pathogen-reduced plasma attenuates the increase in brain water content following traumatic brain injury and hemorrhagic shock in rats the rat.doi/10.1089/neu.2016.4574
  • Volume kinetics of Ringer's lactate solution in acute inflammatory disease10.1016:j.bja.2018.04.023
  • MPO (myeloperoxidase) reduces endothelial glycocalyx thickness dependent on its cationic chargePubmed:29903730
  • Plasma resuscitation improved survival in a cecal ligation and puncture rat model of sepsisPubmed:28591008
  • Experimental models of endotheliopathy: impact of shock severityPubmed:28697004
  • Effects of propranolol and clonidine on brain edema, blood-brain barrier permeability, and endothelial glycocalyx disruption after fluid percussion brain injury in the ratPubmed:28930945
  • The Endothelial Glycocalyx in the Peritoneal Microcirculation of Rats with Chronic Kidney Failure Exposed to Dialysis Solutions173015_06.pdf
  • Plasma ameliorates endothelial dysfunction in burn injuryDoi: 10.1016/j.jss.2018.08.027
  • Dexmedetomidine preserves the endothelial glycocalyx and improves survival in a rat heatstroke modelPubmed: 30374889
  • Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypassPubmed: 30687934
  • Microvascular alterations during cardiac surgery using a heparin or phosphorylcholine coated circuitPubmed: 31787433
  • Increased syndecan-1 and glypican-3 predict poor perinatal outcome and treatment resistance in intrahepatic cholestasis: Syndecan-1, glypican-3 and ICPPubmed: 31919038
  • Heparin Binding Protein and Endothelial Glycocalyx Markers in Severe COVID-19–A Prospective Observational Cohort Study
  • Newly Developed Recombinant Antithrombin Protects the Endothelial Glycocalyx in an Endotoxin-Induced Rat Model of Sepsis33375342
  • Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency34119534
  • The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo …34423832
  • Inhalation of 2% hydrogen improves survival rate and attenuates shedding of vascular endothelial glycocalyx in rats with heat stroke34524269
  • Dexmedetomidine suppresses serum syndecan-1 elevation and improves survival in a rat hemorrhagic shock modelPubmed:35110424
  • Resuscitation of hemorrhagic shock using normal saline does not damage the glycocalyx in the immediate resuscitation phase

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