Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb) Canis familiaris; Canine (Dog) Polyclonal antibody

LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb) Packages (Simulation)
  • Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb) Packages (Simulation)
  • PAA134Ca01.jpg Figure. Western Blot; Sample: Recombinant TNFb, Canine.
  • Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb) Western Blot; Samples: Lane1: Canine Lymph node lysate; Lane2: Rat Spleen lysate; Lane3: Jurkat cell lysate;
    Primary Ab: 0.2µg/ml Rabbit Anti-Canine TNFb Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against TNFb. It has been selected for its ability to recognize TNFb in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • Simvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling processPubMed: 22984252
  • Effectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantationPubmed:26847569
  • Protective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected CytokinesIJST-2328-3548-04-803
  • Effect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a …Pubmed:29305686
  • Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism …Pubmed: 32680553
  • Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort
  • Elevated lymphotoxin-¦Á (TNF¦Â) is associated with intervertebral disc degeneration33441130

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