Biotin-Linked Polyclonal Antibody to Collagen Type X (COL10) Homo sapiens (Human) Labelled antibody

COL10A1; COL10-A1; Schmid Metaphyseal Chondrodysplasia; Collagen Alpha-1(X)chain

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Overview
Properties
  • SourceAntibody labeling
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelBiotin
  • Original Antibody n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Biotin-Linked Polyclonal Antibody to Collagen Type X (COL10) Packages (Simulation)
  • Biotin-Linked Polyclonal Antibody to Collagen Type X (COL10) Packages (Simulation)
  • PAC156Hu71.jpg WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against COL10. It has been selected for its ability to recognize COL10 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

Giveaways

Citations

  • Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor IPubMed: 26123891
  • rAAV-mediated overexpression of sox9, TGF-β and IGF-I in minipig bone marrow aspirates to enhance the chondrogenic processes for cartilage repairPubMed: 26583804
  • Reduced Terminal Complement Complex Formation in Mice Manifests in Low Bone Mass and Impaired Fracture HealingPubmed: 30339839
  • Type X Collagen Assays and Methods of Use Thereof

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