Biotin-Linked Polyclonal Antibody to Heparan Sulfate Proteoglycan (HSPG) Homo sapiens (Human) Labelled antibody

SDC2; HSPG1; SYND2; Fibroglycan; Syndecan 2; Syndecan Proteoglycan 2; Heparan Sulfate Proteoglycan 1,Cell Surface-Associated; Heparan Sulphate Proteoglycan

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Overview
Properties
  • SourceAntibody labeling
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelBiotin
  • Original Antibody RPA565Hu01-Recombinant Heparan Sulfate Proteoglycan (HSPG)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.58mg/ml
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  • Biotin-Linked Polyclonal Antibody to Heparan Sulfate Proteoglycan (HSPG) Packages (Simulation)
  • Biotin-Linked Polyclonal Antibody to Heparan Sulfate Proteoglycan (HSPG) Packages (Simulation)
  • PAA565Hu71.jpg WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HSPG. It has been selected for its ability to recognize HSPG in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Cytotoxic effects of antiglypican-3 against HepG3 cell lineEbscohost: Source
  • Fibrinogen, an endogenous ligand of Toll-like receptor 4, activates monocytes in pre-eclamptic patientsPubmed: 24661950
  • Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzymePubmed: 24530413
  • Evaluation of antiglypican-3 therapy as a promising target for amelioration of hepatic tissue damage in hepatocellular carcinomaPubmed:25449037
  • Impairment of the Endothelial Glycocalyx in Cardiogenic Shock and its Prognostic RelevancePubmed:25692257
  • Endothelial glycocalyx layer shedding following lung resectionpubmed:27643669
  • Circulating syndecans during critical illness.pubmed:28256016
  • Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 andPubmed:29669302
  • Glioma targeting peptide modified apoferritin nanocagePubmed:29726297
  • Postoperative microcirculatory perfusion and endothelial glycocalyx shedding following cardiac surgery with cardiopulmonary bypassPubmed: 30687934
  • Diet Supplementation with a Bioactive Pomace Extract from Olea europaea Partially Mitigates Negative Effects on Gut Health Arising from a Short-Term Fasting Period …
  • Heparin prevents in vitro glycocalyx shedding induced by plasma from COVID-19 patients33781826
  • A novel mutation in ext2 caused hereditary multiple exostoses through reducing the synthesis of heparan sulfate34042151
  • Response of broiler chickens fed diets supplemented with a bioactive olive pomace extract from Olea europaea to an experimental coccidial vaccine?¡­33518110
  • The effect of pre-operative methylprednisolone on postoperative delirium in elderly patients undergoing gastrointestinal surgery: a randomized, double-blind, placebo …34423832

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