Biotin-Linked Polyclonal Antibody to Immunoglobulin G4 (IgG4) Homo sapiens (Human) Labelled antibody

IGHG4; Ig Gamma-4 Chain C Region; Immunoglobulin Heavy Constant Gamma 4; G4m Marker; Immunoglobulin Gm4

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Overview
Properties
  • SourceAntibody labeling
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelBiotin
  • Original Antibody n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Biotin-Linked Polyclonal Antibody to Immunoglobulin G4 (IgG4) Packages (Simulation)
  • Biotin-Linked Polyclonal Antibody to Immunoglobulin G4 (IgG4) Packages (Simulation)
  • Biotin-Linked Polyclonal Antibody to Immunoglobulin G4 (IgG4) Western Blot; Sample: Human Serum
    Primary Ab: 0.2µg/ml Biotin-Linked Polyclonal Antibody to Immunoglobulin G4 (IgG4)
    Second Ab: 0.2µg/ml HRP-Labeled Streptavidin (Catalog: IS089)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against IgG4. It has been selected for its ability to recognize IgG4 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • The Effect of CXC Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingHindawi:Source
  • Utility of serum IgG, IgG4 and carbonic anhydrase II antibodies in distinguishing autoimmune pancreatitis from pancreatic cancer and chronic pancreatitisPubmed:25194335
  • Beneficial effect of T follicular helper cells on antibody class switching of B cells in prostate cancerPubmed:25529861
  • Effect of Cysteamine on Cell Growth and IgG4 Production in Recombinant Sp2.0 CellsPubmed:25561924
  • Loss of interleukin-21 leads to atrophic germinal centers in multicentric Castleman’s diseasePubMed: 26377996
  • The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt SignalingPubMed: 26161394
  • Increasing Foxp3, IgG4 and improvement quality of life after immunotherapy treatment in allergic rhinitis patientsarticle:30785

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