FITC-Linked Polyclonal Antibody to Mucin 5 Subtype B (MUC5B) Mus musculus (Mouse) Labelled antibody

MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin

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Overview
Properties
  • SourceAntibody labeling
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelFITC
  • Original Antibody PAA684Mu01-Polyclonal Antibody to Mucin 5 Subtype B (MUC5B)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • FITC-Linked Polyclonal Antibody to Mucin 5 Subtype B (MUC5B) Packages (Simulation)
  • FITC-Linked Polyclonal Antibody to Mucin 5 Subtype B (MUC5B) Packages (Simulation)
  • PAA684Mu81.jpg Figure. Western Blot; Sample: Recombinant MUC5B, Human.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MUC5B. It has been selected for its ability to recognize MUC5B in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via D-Prostanoid Receptor SignalingJcb: 34199
  • The ERK MAPK/RSK1 cascade phosphorylates CREB to induce MUC5B gene expression via DP1 receptor signalingJcb: 247684
  • Pyocyanin-induced mucin production is associated with redox modification of FOXA2PubMed: PMC3765780
  • Secreted mucins and airway bacterial colonization in non-CF bronchiectasisPubMed: 26172851
  • Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsivenessdoi:10.1164
  • Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonizationdoi:10.1513
  • Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой10.20538/1682-0363-2017-2-146-158
  • Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polypsPubmed:29802623
  • Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signalingPubmed:29382534
  • インフルエンザ菌Phosphorylcholine の表出と中耳ムチンMUC5AC 及びMUC5B 産生への影響10.5648:jjiao.36.7
  • The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung diseasePubmed: 30573494
  • ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanismPubmed: 31278375

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