Monoclonal Antibody to Calreticulin (CALR)

CRT; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 118.00 US$ 274.00 US$ 392.00 US$ 980.00 US$ 3,920.00
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Overview

Properties

Product No.MAB486Ra23
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction Metabolic pathway Bone metabolism

SourceMonoclonal antibody preparation, Host Mouse
Ig Isotype IgG1 Kappa, Clone Number C4
PurificationProtein A + Protein G affinity chromatography
LabelNone
Immunogen RPB486Ra01-Recombinant Calreticulin (CALR)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 1mg/ml

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Rat Colon Tissue; Primary Ab: 30µg/ml Mouse Anti-Rat CALR Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Cardiac Muscle Tissue;
Primary Ab: 30µg/ml Mouse Anti-Rat CALR Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Testis Tissue;
Primary Ab: 30µg/ml Mouse Anti-Rat CALR Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Liver Tissue;
Primary Ab: 30µg/ml Mouse Anti-Rat CALR Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Rat Stomach Tissue;
Primary Ab: 30µg/ml Mouse Anti-Rat CALR Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)

Western Blot; Sample: Hela cell lysate Primary Ab: 0.2µg/ml Mouse Anti-Rat CALR Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CALR. It has been selected for its ability to recognize CALR in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human)

Giveaways

Increment services

Citations

Oxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of MelanocytesPubmed: 23771121
The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKCMdpi: Source
Amniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranesPubmed:26953684
Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes.pubmed:28152632
Investigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modelingDoi: 10.1016/j.talanta.2018.11.098
Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemiaPubmed:35171673