Monoclonal Antibody to Cathepsin D (CTSD) Homo sapiens (Human) Monoclonal antibody

CPSD; CLN10; Lysosomal Aspartyl Protease; Ceroid-Lipofuscinosis,Neuronal 10

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number C7
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen EPB280Hu61-Eukaryotic Cathepsin D (CTSD)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Cathepsin D (CTSD) Packages (Simulation)
  • Monoclonal Antibody to Cathepsin D (CTSD) Packages (Simulation)
  • Monoclonal Antibody to Cathepsin D (CTSD) Western Blot; Samples: Lane1: HepG2 cell lysate; Lane2: MCF7 cell lysate; Lane3: A431 cell lysate;
    Primary Ab: 0.3µg/ml Mouse Anti-Human CTSD Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cathepsin D (CTSD) Western Blot; Sample: Rat Kidney lysate
    Primary Ab: 0.3µg/ml Mouse Anti-Human CTSD Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CTSD. It has been selected for its ability to recognize CTSD in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitroScienceDirect: S0161589011008297
  • Plasma Cathepsin D Levels: A Novel Tool to Predict Pediatric Hepatic InflammationPubMed: 25732418
  • Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to interventionpubmed:27922112
  • Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanismspubmed:28061403
  • Limited applicability of cathepsin D for the diagnosis and monitoring of non‐alcoholic steatohepatitis
  • Plasma cathepsin D activity is negatively associated with hepatic insulin sensitivity in overweight and obese humansPubmed: 31690989
  • Iron and Advanced Glycation End Products: Emerging Role of Iron in Androgen Deficiency in ObesityPubmed: 32235809
  • LVV-hemorphin-7 (LVV-H7) plays a role in antinociception in a rat model of alcohol-induced pain disorders33253777
  • Identification of Cathepsin D as a Plasma Biomarker for Alzheimer's Disease33445607
  • Analysis of silymarin-modulating effects against acrylamide-induced cerebellar damage in male rats: Biochemical and pathological markers33965515
  • PGRS Domain of Rv0297 of Mycobacterium tuberculosis Is Involved in Modulation of Macrophage Functions to Favor Bacterial Persistence33042856
  • Serum CathepsinD in pregnancy: relation with metabolic and inflammatory markers and effects of fish oils and probioticsPubmed:35304048
  • A Comparison of Various Chips Used for the Manufacture of Biosensors Applied in Non-Fluidic Array SPRi, Based on the Example of Determination of Cathepsin DPubmed:35049649

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