Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) Homo sapiens (Human) Monoclonal antibody

IPOA1; QIP2; RCH1; SRP1alpha; RAG Cohort 1; Importin Alpha 1; RAG cohort protein 1; SRP1-alpha

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number C10
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPE748Hu01-Recombinant Karyopherin Alpha 2 (KPNa2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) Packages (Simulation)
  • Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) Packages (Simulation)
  • Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) IHC Images
  • Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) FITC staining on IF;
    Sample: HepG2 cell
    Primary Ab: 20µg/ml Mouse Anti-Human KPNa2 Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu11)
  • MAE748Hu21.jpg Figure. Western Blot; Sample: Recombinant KPNa2, Human.
  • Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) Western Blot; Sample: Human 293T cell lysate;
    Primary Ab: 2µg/ml Mouse Anti-Human KPNa2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) Western Blot; Sample: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: Raji cell lysate; Lane4: U2OS cell lysate
    Primary Ab: 0.4µg/ml Mouse Anti-Human KPNa2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) Human A549 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human KPNa2 Monoclonal Antibody (Catalog MAE748Hu21, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against KPNa2. It has been selected for its ability to recognize KPNa2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL;
Immunocytochemistry: 5-30µg/mL;
Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • KPNA2 is a promising biomarker candidate for esophageal squamous cell carcinoma and correlates with cell proliferationPubmed:25109899
  • KPNA2 is a potential diagnostic serum biomarker for epithelial ovarian cancer and correlates with poor prognosispubmed:28651492
  • Association of overexpressed karyopherin alpha 2 with poor survival and its contribution to interleukin‐1β‐induced matrix metalloproteinase expression in oral cancerPubmed:29542209
  • Nuclear transport proteins are secreted by cancer cells and identified as potential novel cancer biomarkers34591985

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