Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) Homo sapiens (Human) Monoclonal antibody

IPOA1; QIP2; RCH1; SRP1alpha; RAG Cohort 1; Importin Alpha 1; RAG cohort protein 1; SRP1-alpha

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number C19
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPE748Hu01-Recombinant Karyopherin Alpha 2 (KPNa2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) Packages (Simulation)
  • Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) Packages (Simulation)
  • Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) DAB staining on IHC-P; Sample: Human Colon Tissue; Primary Ab: 10µg/ml Mouse Anti-Human KPNa2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Karyopherin Alpha 2 (KPNa2) DAB staining on IHC-P;
    Sample: Human Colon Tissue
    Primary Ab: 10µg/ml Mouse Anti-Human KPNa2 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • MAE748Hu25.jpg Western Blot; Sample: 293T cell lysate Primary Ab: 2µg/ml Mouse Anti-Human KPNa2 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against KPNa2. It has been selected for its ability to recognize KPNa2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • KPNA2 is a promising biomarker candidate for esophageal squamous cell carcinoma and correlates with cell proliferationPubmed:25109899
  • KPNA2 is a potential diagnostic serum biomarker for epithelial ovarian cancer and correlates with poor prognosispubmed:28651492
  • Association of overexpressed karyopherin alpha 2 with poor survival and its contribution to interleukin‐1β‐induced matrix metalloproteinase expression in oral cancerPubmed:29542209
  • Nuclear transport proteins are secreted by cancer cells and identified as potential novel cancer biomarkers34591985

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