Monoclonal Antibody to Cytokeratin 18 (CK18) Homo sapiens (Human) Monoclonal antibody

KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number E4
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB231Hu01-Recombinant Cytokeratin 18 (CK18)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Cytokeratin 18 (CK18) Packages (Simulation)
  • Monoclonal Antibody to Cytokeratin 18 (CK18) Packages (Simulation)
  • Monoclonal Antibody to Cytokeratin 18 (CK18) DAB staining on IHC-P; Sample: Human Cerebrum Tissue; Primary Ab: 10µg/ml Mouse Anti-Human KRT18 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cytokeratin 18 (CK18) DAB staining on IHC-P;
    Sample: Human Lung cancer Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human KRT18 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cytokeratin 18 (CK18) DAB staining on IHC-P;
    Sample: Human Endometrial cancer Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human KRT18 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cytokeratin 18 (CK18) DAB staining on IHC-P;
    Sample: Human Liver cancer Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human KRT18 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cytokeratin 18 (CK18) DAB staining on IHC-P;
    Sample: Human Breast cancer Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human KRT18 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cytokeratin 18 (CK18) FITC staining on IF;
    Samples: Human HepG2 cell;
    Primary Ab: 20µg/ml Mouse Anti-Human KRT18 Antibody
    Second Ab: 1.5µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu18)
  • MAB231Hu21.jpg Figure. Western Blot; Sample: Recombinant KRT18, Human.
  • Monoclonal Antibody to Cytokeratin 18 (CK18) Western Blot; Sample: Rat Liver lysate
    Primary Ab: 3µg/ml Mouse Anti-Human KRT18 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cytokeratin 18 (CK18) Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human CK18 Monoclonal Antibody (Catalog MAB231Hu21, red histogram) or Isotype control antibody (Catalog IS067-Mu03, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CK18. It has been selected for its ability to recognize CK18 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Flow cytometry: 10µg/ml;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosisPubmed:27186801
  • Cigarette Smoke-Induced Alterations in Frontal White Matter Lipid Profiles Demonstrated by MALDI-Imaging Mass Spectrometry: Relevance to Alzheimer's DiseasePubmed:26836183
  • Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring
  • Lipidomic‐based investigation into the therapeutic effects of PPC and BBD on rats with NAFLD34727379
  • Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …Pubmed:35212074

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