Monoclonal Antibody to Mucin 5 Subtype B (MUC5B)
MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin
Overview
Properties
- Product No.MAA684Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only - DownloadInstruction Manual
- CategoryTumor immunityInfection immunityPulmonologyDermatology
- SourceMonoclonal antibody preparation, Host Mouse
- Ig Isotype IgG2b Kappa, Clone Number C10
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPA684Hu02-Recombinant Mucin 5 Subtype B (MUC5B)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid, Concentration 1mg/ml
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Specifity
The antibody is a mouse monoclonal antibody raised against MUC5B. It has been selected for its ability to recognize MUC5B in immunohistochemical staining and western blotting.
Usage
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Storage
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stability
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Giveaways
Increment services
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
Citations
- The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via D-Prostanoid Receptor SignalingJcb: 34199
- The ERK MAPK/RSK1 cascade phosphorylates CREB to induce MUC5B gene expression via DP1 receptor signalingJcb: 247684
- Pyocyanin-induced mucin production is associated with redox modification of FOXA2PubMed: PMC3765780
- Secreted mucins and airway bacterial colonization in non-CF bronchiectasisPubMed: 26172851
- Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsivenessdoi:10.1164
- Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonizationdoi:10.1513
- Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой10.20538/1682-0363-2017-2-146-158
- Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polypsPubmed:29802623
- Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signalingPubmed:29382534
- インフルエンザ菌Phosphorylcholine の表出と中耳ムチンMUC5AC 及びMUC5B 産生への影響10.5648:jjiao.36.7
- The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung diseasePubmed: 30573494
- ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanismPubmed: 31278375