KO-Validated
Polyclonal Antibody to Exportin 1 (XPO1)

Exp1; CRM1 Homolog,Yeast; Chromosome region maintenance 1 protein homolog

UOM
1. 20µl
2. 100µl
3. 200µl
4. 1ml
5. 10ml
FOB US$ 105.00 US$ 245.00 US$ 350.00 US$ 875.00 US$ 3,500.00
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Overview

Properties

Product No.PAC258Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
ApplicationsWB; IHC; FCM
If the antibody is used in flow cytometry, please check FCM antibodies.
Research use only
DownloadInstruction Manual
CategorySignal transduction Infection immunity Immunodeficiency

SourcePolyclonal antibody preparation, Host Rabbit
Ig Type IgG, Potency n/a
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPC258Hu01-Recombinant Exportin 1 (XPO1)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid, Concentration 0.5mg/mL

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Packages (Simulation)
Packages (Simulation)

DAB staining on IHC-P; Sample: Human Colon Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Uterus Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Small intestine Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lymph node Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Stomach Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Pancreas Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Spinal cord Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Spleen Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant XPo1, Human.
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: K562 cell lysate; Lane3: Human Lung lysate; Lane4: Porcine Small intestine lysate
Primary Ab: 1µg/ml Rabbit Anti-Human XPo1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: XPo1 knockout Hela cell lysate;
Predicted MW: 123kd
Observed MW: 110kd
Primary Ab: 1µg/ml Rabbit Anti-Human XPO1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Human K562 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human XPo1 Polyclonal Antibody (Catalog PAC664Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).

ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against XPO1. It has been selected for its ability to recognize XPO1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Flow cytometry: 20μg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Increment services

Citations

Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid LeukemiaPubMed: 26408402
Inactivation of the p53–KLF8–CEBPA Axis in Acute Myeloid LeukemiaPubMed: 26408402
Mahendra Seervi, S. Sumi, Aneesh Chandrasekharan, Abhay K. Sharma &Doi: 10.1007/s13402-019-00451-1
Molecular profiling of anastatic cancer cells: potential role of the nuclear export pathwayPubmed: 31147963
XPO1 Gene Therapy Attenuates Cardiac Dysfunction in Rats with Chronic Induced Myocardial InfarctionPubmed: 31768947
Identification of intracellular glycosaminoglycan-interacting proteins by affinity purification mass spectrometry34472763

KO-Validated Polyclonal Antibody to Exportin 1 (XPO1)