Polyclonal Antibody to High Temperature Requirement Factor A1 (HTRA1) Escherichia coli Polyclonal antibody

HtrA; L56; ORF480; PRSS11; HtrA Serine Peptidase 1; Protease,Serine,11; IGFBP5-Protease; High-temperature requirement A serine peptidase 1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPL604Co02-Recombinant High Temperature Requirement Factor A1 (HTRA1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to High Temperature Requirement Factor A1 (HTRA1) Packages (Simulation)
  • Polyclonal Antibody to High Temperature Requirement Factor A1 (HTRA1) Packages (Simulation)
  • PAL604Co02.jpg Figure. Western Blot; Sample: Recombinant HTRA1, Escherichia coli.
  • Polyclonal Antibody to High Temperature Requirement Factor A1 (HTRA1) Western Blot; Sample: E.coli lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Coli HTRA1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against HTRA1. It has been selected for its ability to recognize HTRA1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Alterations of maternal plasma HTRA1 level in preeclampsia complicated by IUGRPubMed: 23083999
  • Upregulation of HtrA4 in the placentas of patients with severe pre-eclampsiaPubMed: 22964307
  • Association of frailty with the serine protease HtrA1 in older adultsPubmed:27058767
  • HtrA1 up-regulates expression of MMPs via Erk1/2/Rock-dependent pathwaysfiles:ijcep0069706.pdf
  • Association between first trimester plasma htra1 level and subsequent preeclampsia: a possible early marker?
  • HtrA1 upregulates the expression of ADAMTS-5 in HNPCs via the ERK/NF-κB/JNK signaling pathwayPubmed: 31497227
  • First trimester HtrA1 maternal plasma level and spontaneous preterm birthPubmed: 32102578
  • Cross-sectional associations among P3NP, HtrA, Hsp70, Apelin and sarcopenia in Taiwanese population33743591

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