Polyclonal Antibody to Mucin 1 (MUC1) Mus musculus (Mouse) Polyclonal antibody

CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; CA153; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA413Mu03-Recombinant Mucin 1 (MUC1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Mucin 1 (MUC1) Packages (Simulation)
  • Polyclonal Antibody to Mucin 1 (MUC1) Packages (Simulation)
  • Polyclonal Antibody to Mucin 1 (MUC1) DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse MUC1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mucin 1 (MUC1) DAB staining on IHC-P;
    Samples: Mouse Kidney Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse MUC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mucin 1 (MUC1) DAB staining on IHC-P;
    Samples: Mouse Heart Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse MUC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mucin 1 (MUC1) DAB staining on IHC-P;
    Samples: Mouse Lung Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse MUC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mucin 1 (MUC1) DAB staining on IHC-P;
    Samples: Mouse Uterus Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse MUC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mucin 1 (MUC1) DAB staining on IHC-P;
    Samples: Mouse Spleen Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse MUC1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Mucin 1 (MUC1) DAB staining on IHC-P;
    Sample: Mouse Uterus Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse MUC1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA413Mu03.jpg Figure. Western Blot; Sample: Recombinant MUC1, Mouse.
  • Polyclonal Antibody to Mucin 1 (MUC1) Western Blot; Sample: Mouse Pancreas lysate
    Primary Ab: 3µg/ml Rabbit Anti-Mouse MUC1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MUC1. It has been selected for its ability to recognize MUC1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.PubMed: 20717734
  • Host Defense Proteins in Breast Milk and Neonatal Yeast ColonizationPubMed: 26116637
  • Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive IndicatorPubMed: 26446295
  • The Presence and Anti-HIV-1 Function of Tenascin C in Breast Milk and Genital FluidsPubmed:27182834
  • The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghaifiles:ijcem0020163.pdf
  • Ultrasensitive detection of mucin 1 biomarker by immuno-loop-mediated isothermal amplification.pubmed:28107977
  • An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489
  • Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA10.21769:BioProtoc.2879
  • Aptasensors as a new sensing technology developed for the detection of MUC1 mucin: A review
  • A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample

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