Polyclonal Antibody to Occludin (OCLN) Mus musculus (Mouse) Polyclonal antibody

Tight Junction Protein Occludin TM4 Minus

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC228Mu02-Recombinant Occludin (OCLN)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/mL
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  • Polyclonal Antibody to Occludin (OCLN) Packages (Simulation)
  • Polyclonal Antibody to Occludin (OCLN) Packages (Simulation)
  • PAC228Mu02.jpg Figure. Western Blot; Sample: Recombinant OCLN, Mouse.
  • Polyclonal Antibody to Occludin (OCLN) Western Blot; Sample: Lane1: Mouse Cerebrum lysate; Lane2: Mouse Liver lysate
    Primary Ab: 1μg/ml Rabbit Anti-Mouse OCLN Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Occludin (OCLN) Western Blot; Sample: Lane1: Mouse Cerebrum lysate; Lane2: Mouse Cerebellum lysate; Lane3: Rat Cerebrum lysate; Lane4: Rat Cerebellum lysate; Lane5: Mouse Lung lysate
    Primary Ab: 0.2µg/ml Rabbit Anti-Mouse OCLN Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Occludin (OCLN) Western Blot; Sample: HUH7 cell lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Mouse OCLN Antibody
    Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against OCLN. It has been selected for its ability to recognize OCLN in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • HIV-1 Tat protein increases the permeability of brain endothelial cells by both inhibiting occludin expression and cleaving occludin via matrix metalloproteinase-9ScienceDirect: S0006899311021408
  • Disruption of the Circadian Clock in Mice Increases Intestinal Permeability and Promotes Alcohol-Induced Hepatic Pathology and InflammationPubMed: PMC3688973
  • A new panel of blood biomarkers for the diagnosis of mild traumatic brain injury/concussion in adultsPubMed: 25794137
  • Methods for Diagnosis and Treatment of Concussion or Brain Injury2016:0103140
  • Blood Occludin Level as a Potential Biomarker for Early Blood Brain Barrier Damage Following Ischemic Strokearticles:srep40331
  • Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal …Pubmed:29375374
  • Fas activation alters tight junction proteins in acute lung injuryPubmed: 30385692
  • The Effect of Dietary Quercetin on the Glutathione Redox System and Small Intestinal Functionality of Weaned PigletsPubmed: 31426309
  • Peripheral blood occludin level as a biomarker for preoperative cerebral edema in patients with brain tumorsPubmed: 32884584
  • Intestinal Permeability in Participants With Thermal Injury: A Case Series From a Prospective, Longitudinal Study (HESTIA)
  • A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and …34492054
  • Higher serum occludin after successful reperfusion Is associated with early neurological deteriorationPubmed:35338575
  • Impact de l'incorporation d'acides gras à chaine moyenne, de suppléments de levures et d'avoine nue sur la santé intestinale ainsi que sur la consommation …

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